CFDA-SE

CFDA-SE

Author: ApexBio

spu:

C3430

CAS:

150347-59-4

purity:

0.98

shortsummary:

A stable, cell-permeable dye commonly used in cell proliferation assay

204005-46-9 References

Formula:

C29H19NO11

M.Wt:

557.46

Chemical Name:

3',6'-bis(acetyloxy)-3-oxo-2,5-dioxo-1-pyrrolidinyl ester-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid

SMILES:

CC(OC1=CC2=C(C3(C4=C(C=C(OC(C)=O)C=C4)O2)C5=C(C(O3)=O)C=C(C(ON6C(CCC6=O)=O)=O)C=C5)C=C1)=O.CC(OC7=CC8=C(C9(C%10=C(C(O9)=O)C=CC(C(ON%11C(CCC%11=O)=O)=O)=C%10)C%12=C(C=C(OC(C)=O)C=C%12)O8)C=C7)=O

Solubility:

≥37.17 mg/mL in DMSO with ultrasonic; insoluble in EtOH; insoluble in H2O

Storage:

Store at -20°C

DeliveryTime:

In stock

tsa chemical

ShippingType:

Blue ice

description:

CFDA-SE (i.e. carboxyfluorescein diacetate succinimidyl ester) is a stable, cell-permeable dye, composed of a fluorescein molecule containing two acetate moieties and a succinimidyl ester functional group. After diffusion into the intracellular environment, cellular esterases remove the acetyl moieties from CFDA-SE, leaving behind CFSE (i.e. carboxyfluorescein succinimidyl ester) which covalently binds to amino groups on intracellular macromolecules by its succinimidyl group and can be retained within the cells for at least 8 weeks. CFSE stained cells can then be detected by fluorescence microscopy and flow cytometry under the excitation/emission maxima of 491/518 nm. CFDA-SE provides a valuable tool to quantify cell division and track cell migration, due to the sequential decrease in fluorescent labeling in daughter cells.

related websites:

APExBIO Technology LLC is a premier provider of Small Molecule Inhibitors/Activators, Compound Libraries, Peptides, Assay Kits, Fluorescent Labels, Enzymes, Modified Nucleotides, mRNA synthesis and various tools for Molecular Biology. We carry a broad product line in over 15711 different research areas such as cancer, immunology, neurosciences, apoptosis and epigenetics etc. Based in USA (Houston, Texas), we have been serving the needs of customers across the world.